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KMID : 0359320220620040001
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Korean Journal of Veterinary Research
2022 Volume.62 No. 4 p.1 ~ p.1
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Serological responses and protection levels in chickens administered with Newcastle disease vaccines
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Seung Geum-Ji
Kim Ji-Ye
Kim Hyo-Bi
Kim Ji-Yeon
Jang Yang-Ho
Kim Yeon-Hee
Her Moon
Yi Seong-Joon
Lee Keun-Woo
Jang Il
Lee Young-Ju
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Abstract
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Vaccination against Newcastle disease (ND) is the most effective means of controlling the disease, and these vaccines are commercialized only after their safety and effectiveness have been verified through tests that comply with Korean Standards of National Lot Release for Veterinary Biologics. This study investigated whether a relatively convenient and safe serological test can be used in place of the challenge test using highly virulent ND virus. Hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA) were considered positive of log2 2 or more and cutoff value of 200 or more, respectively, in both live and inactivated vaccines. However, when the antibody levels of the live and inactivated vaccines induced using the Ulster 2C, KBNP-C4152R2L, and K148/08 strains were compared, the antibody titers for inactivated vaccines were significantly higher than those for live vaccines in both the HI assay and ELISA. A strong positive correlation was observed between HI and ELISA antibody titers. The live vaccines corresponded to a survival rates of ≥ 80% and the inactivated vaccines corresponded to 100% survival rates. This study confirmed that standard efficacy tests can serve as serological tests, and can replace the challenge test and that the vaccine approval process can be improved.
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KEYWORD
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Newcastle disease, serological tests, Newcastle disease virus, Vaccine efficacy, vaccines
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